E0771/GFP-luciferase cells are derived from the mouse mammary adenocarcinoma cell line E0771 by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. E0771 cells are frequently used as a preclinical model for studying breast cancer, particularly in the context of tumor biology, metastasis, and therapeutic interventions. E0771/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.