DU145/GFP cells are derived from the human prostate cancer cell line DU145 by stably integration of a constitutive GFP stably expression construct. DU145 cells represent an aggressive and androgen-independent form of prostate cancer, which is often resistant to standard hormone-based therapies. DU145 cell line has been used as a model for identifying the molecular mechanisms underlying prostate cancer progression and metastasis, as well as potential therapeutic targets for the treatment of advanced prostate cancer. DU145/GFP cells stably express GFP, can be used for in vitro assays and in vivo imaging.