CT26.WT/iRFP cells are derived from the mouse colon carcinoma CT26.WT cell line by stably integration of a constitutive miRFP670 stably expression construct. The CT26.WT cell line has been widely used in cancer research and drug development. CT26.WT/iRFP cells stably express miRFP670, can be used for in vitro assays and in vivo imaging.