Colo 320DM/iRFP cells are derived from the human colorectal adenocarcinoma Colo 320DM cell line by stably integration of a constitutive miRFP670 stably expression construct. The Colo 320DM cell line has been widely used in cancer research and drug development. Colo 320DM/iRFP cells staby stably express miRFP670, can be used for in vitro assays and in vivo imaging.