Colo 205/GFP cells are derived from the human colorectal adenocarcinoma Colo 205 cell line by stably integration of a constitutive GFP stably expression construct. The Colo 205 cell line has been widely used in cancer research and drug development. Colo 205/GFP cells stably stably express GFP, can be used for in vitro assays and in vivo imaging.