CCRF-CEM/GFP-luciferase cells are derived from the human CCRF-CEM T lymphoblast cell line by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. CCRF-CEM cell line was generated from acute lymphoblastic leukemia, has been widely used for investigating the mechanisms underlying leukemia development, progression, and drug resistance. Additionally, these cells are often employed in screening assays to evaluate the efficacy and toxicity of potential anti-leukemic drugs. CCRF-CEM/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.