CAMKII-GFP (Puro) lentivirus expresses GFP under the control of the 1.3kb murine α-Calcium/calmodulin-dependent kinase II (CaMKII) promoter, with puromycin as a selection marker. CaMKII is a serine/threonine protein kinase with expression restricted to excitatory neurons in the neocortex and hippocampus. CaMKII is involved in many signaling cascades that important to learning and memory. CAMKII-GFP (Puro) lentivirus is ready-for-use to label excitatory neurons in the neocortex and hippocampus and study CAMKII promoter regulation.