BW5147.G.1.4/Luciferase cells are derived from the mouse T cell lymphoma BW5147.G.1.4 cell line by stably integration of a constitutive Firefly luciferase stably expression construct. BW5147.G.1.4 cells have been used in cancer research and drug development. BW5147.G.1.4/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.