ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1) is a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. ASCL1 lentivirus (TET-inducible, Puro) expresses human ASCL1 under the control of the Tet responsive promoter, with puromycin as a selection marker.