To order
Overview
Adeno-associated virus (AAV) vector is among the most commonly used vectors for in vivo gene therapy. AAV vector stands out by its safety profile. Infection of AAV vector is not pathogenic, it does not integrate into the host genome and instead forms episomal concatemers in the cell nucleus, therefore will not generate integration related mutagenesis. AAV vectors demonstrate high transduction efficiency to both dividing and non-dividing cells, stable long-term expression, low immunogenicity and selective tissue tropism.
FenicsBIO provides superior quality, fast turnaround AAV production service to meet your AAV-based research needs. We have developed proprietary reagents and technologies to cover every step of AAV production, including construct generation, AAV packaging, purification and storage.
Features:
- One-stop cloning service, can start from scratch
- Fast turnaround, turnaround in 2-3 weeks
- Project update weekly, and Q/C reports in every milestone.
Deliverables/Lead Time
| SKU No. | Production scale | Deliverables | Purity grade | Titration | Lead time |
| C-AAV-11 | >1x10^11 GC/ml, 200ul | AAV particles, 4x50ul | In vitro | Real-time PCR | 2-3 weeks |
| C-AAV-12 | >1x10^12 GC/ml, 1ml | AAV particles, 5x200ul | In vitro | Real-time PCR | 2-3 weeks |
| C-AAV-13 | >1x10^13 GC/ml, 500ul | AAV particles, 5x100ul | In vitro and In vivo | Real-time PCR | 2-3 weeks |
Note: If construct generation is required, another 1-2 weeks are needed for most constructs.
QC Testing
The Q/C testing for FenicsBIO’s Custom AAV Production Service includes:
- Restriction digestion pattern verification and sequence confirmation for the AAV constructs if generated by FenicsBIO
- AAV viral particles sterility testing, titration by Real-time PCR
Project Initiation
Phase I: Project information collection
Our scientists will work with you to figure out the details of your AAV production requirement, including AAV titer, volume and the serotype, whether you need us to generate the AAV constructs and if you do, what requirements you have for the constructs, including the promoter to use, selection marker and reporter requirements.
Phase II: Project plan approval
Based on the above information you provided, we will formulate a project plan and send you a quotation. After you approve the project plan, you can use the quotation to place an order.
Phase III: Starting material shipment
If you have AAV construct, please send at least 1 ug of the plasmid, bacterial stab or glycerol stock. In most cases, these starting materials can be shipped at room temperature.
If you need us to generate AAV constructs, your project manager will send you a list of starting material needed.
If you have any questions or need more information, please Contact Us by email or phone, we will get back to you in 24 hours.