A549/Luciferase cells are derived from the human A549 lung carcinoma cell line by stably integration of a constitutive Firefly luciferase stably expression construct. A549 cell line has been widely used in cancer research, immunological studies and drug development. A549/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.