A375/GFP-luciferase cells are derived from the human melanoma A375 cell line by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. A375 cell line has been widely used in cancer research and drug development. A375/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.