22Rv1/iRFP cells are derived from the human prostate cancer 22Rv1 cell line by stably integration of a constitutive miRFP670 stably expression construct. 22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line stably expresses prostate specific antigen (PSA). 22Rv1 cell line has been widely used in cancer research and drug development. 22Rv1/iRFP cells stably express miRFP670, can be used for in vitro assays and in vivo imaging.