Near-infrared fluorescent proteins iRFP and Firefly luciferase are powerful tools for in vivo imaging. Due to its red-shifted excitation/emission spectrum, iRFP has much lower tissue background than the other fluorescent proteins when used for in vivo imaging. This iRFP-luciferase lentivirus coexpresses miRFP670 and Firefly luciferase under the control of the UBC promoter, with puromycin as a selection marker.